Standard Wide-Field Fluorescence Compared to Structured Illumination

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Created 03/07/2006

In these images of bovine pulmonary artery endothelial cells, the mitochondria are stained with MitoTracker Red CMXRos (red), and F-actin is stained with BODIPY FL phallacidin (green). On the left is a standard wide-field fluorescence image that includes signal fluorescence from above and below focus. On the right is the same image following deconvolution using the Zeiss Apotome Structured Illumination System.

Credits

• Jon Ekman , ITG, Beckman Institute

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